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| PROMOTER RESOURCES PAGE |
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Promoter: a region of DNA usually occurring upstream from a gene coding region and acting as a controlling element in the expression of that gene. It serves as a recognition signal for an RNA polymerase and marks the site of initiation of transcription. |
| Figure: An overview of promoter isolation strategy: |
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Promoters can be predicted by using variety of programs. (Click here) Before we proceed to isolate a promoter, the first thing one would like to know is the site of initiation of transcription which is commonly referred to as "Transcription Start Site". |
| 1. Transcription Start Site mapping |
| 2. Making deletion constructs |
| 3. Cloning / Sub-cloning |
| 4. Transfections |
| 5. Luciferase assays |
| 6. Data plotting |
| 7. Data interpretation
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Last Updated 11-01-2004 |
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